Skip to main content

Novasign’s publications

Find our publications in the fields of upstream and downstream processing.

Upstream processing

  1. End-to-end continuous E. coli biomanufacturing of recombinant proteins via integrated and automated digital control (opens in a new tab)

    Microbial Cell Factories, 2026 · In collaboration with enGenes, Qubicon, SimVantage, Tosoh Bioscience, BOKU · doi:10.1186/s12934-026-03127-2

    Simon F, Mairhofer J (and 17 more authors)

  2. Digital Model-Driven Cell & Gene Therapy Process Development (opens in a new tab)

    Biotechnology and Bioengineering 122(12), 3271–3284, 2025 · In collaboration with Antleron · doi:10.1002/bit.70051

    Claes E, Schrooten J (and 7 more authors)

  3. Seed Train Optimization in Microcarrier-Based Cell Culture Post In Situ Cell Detachment through Scale-Down Hybrid Modeling (opens in a new tab)

    Bioengineering 11(3), 268, 2024 · In collaboration with FH Campus Wien · doi:10.3390/bioengineering11030268

    Ebrahimian A, Kühnel H (and 4 more authors)

  4. Parameter Optimization and Capacitance-Based Monitoring of In Situ Cell Detachment in Microcarrier Cultures (opens in a new tab)

    Processes 12(9), 1887, 2024 · In collaboration with FH Campus Wien · doi:10.3390/pr12091887

    Ebrahimian A, Kühnel H (and 4 more authors)

  5. Comparison of mechanistic and hybrid modeling approaches for characterization of a CHO cultivation process: Requirements, pitfalls and solution paths (opens in a new tab)

    Biotechnology Journal 18(1), 2200381, 2023 · In collaboration with Hamburg University of Technology · doi:10.1002/biot.202200381

    Bayer B, Möller J (and 2 more authors)

  6. Fundamental insights in early-stage inclusion body formation (opens in a new tab)

    Microbial Biotechnology 16(5), 893–900, 2023 · In collaboration with TU Wien · doi:10.1111/1751-7915.14117

    Kopp J, Spadiut O (and 4 more authors)

  7. Boost dynamic protocols for producing mammalian biopharmaceuticals with intensified DoE—a practical guide to analyses with OLS and hybrid modeling (opens in a new tab)

    Frontiers in Chemical Engineering 4, 1044245, 2023 · In collaboration with Boehringer Ingelheim · doi:10.3389/fceng.2022.1044245

    Nold V, Knapp BL (and 9 more authors)

  8. Proton-transfer-reaction mass spectrometry (PTR-MS) for online monitoring of glucose depletion and cell concentrations in HEK 293 gene therapy processes (opens in a new tab)

    Biotechnology Letters 44(1), 77–88, 2022 · In collaboration with Takeda · doi:10.1007/s10529-021-03205-y

    Bayer B, Hernandez Bort JA (and 7 more authors)

  9. Model Transferability and Reduced Experimental Burden in Cell Culture Process Development Facilitated by Hybrid Modeling and Intensified Design of Experiments (opens in a new tab)

    Frontiers in Bioengineering and Biotechnology 9, 740215, 2021 · In collaboration with Bilfinger Life Science · doi:10.3389/fbioe.2021.740215

    Bayer B, Sissolak B (and 2 more authors)

  10. Digital Twin Application for Model-Based DoE to Rapidly Identify Ideal Process Conditions for Space-Time Yield Optimization (opens in a new tab)

    Processes 9(7), 1109, 2021 · In collaboration with BOKU · doi:10.3390/pr9071109

    Bayer B, Duerkop M (and 3 more authors)

  11. The shortcomings of accurate rate estimations in cultivation processes and a solution for precise and robust process modeling (opens in a new tab)

    Bioprocess and Biosystems Engineering 43(2), 169–178, 2020 · In collaboration with Bilfinger Life Science · doi:10.1007/s00449-019-02214-6

    Bayer B, Striedner G (and 3 more authors)

  12. Comparison of Modeling Methods for DoE‐Based Holistic Upstream Process Characterization (opens in a new tab)

    Biotechnology Journal 15(5), 1900551, 2020 · In collaboration with BOKU · doi:10.1002/biot.201900551

    Bayer B, Duerkop M (and 2 more authors)

  13. Hybrid Modeling and Intensified DoE: An Approach to Accelerate Upstream Process Characterization (opens in a new tab)

    Biotechnology Journal 15(9), 2000121, 2020 · In collaboration with BOKU · doi:10.1002/biot.202000121

    Bayer B, Striedner G, Duerkop M

  14. Soft sensor based on 2D‐fluorescence and process data enabling real‐time estimation of biomass in Escherichia coli cultivations (opens in a new tab)

    Engineering in Life Sciences 20(1–2), 26–35, 2020 · In collaboration with BOKU · doi:10.1002/elsc.201900076

    Bayer B, Striedner G (and 3 more authors)

Downstream processing

  1. Enabling continuous plasmid DNA production: Model-based optimization of inline alkaline lysis (opens in a new tab)

    Biochemical Engineering Journal 235, 110345, 2026 · In collaboration with Qubicon, enGenes · doi:10.1016/j.bej.2026.110345

    Korn R, Mairhofer J (and 10 more authors)

  2. End-to-end continuous E. coli biomanufacturing of recombinant proteins via integrated and automated digital control (opens in a new tab)

    Microbial Cell Factories, 2026 · In collaboration with enGenes, Qubicon, SimVantage, Tosoh Bioscience, BOKU · doi:10.1186/s12934-026-03127-2

    Simon F, Mairhofer J (and 17 more authors)

  3. Hybrid modeling reduces experimental effort to predict performance of serial and parallel single-pass tangential flow filtration (opens in a new tab)

    Separation and Purification Technology 276, 119277, 2021 · In collaboration with BOKU · doi:10.1016/j.seppur.2021.119277

    Krippl M, Dürauer A (and 2 more authors)

  4. Hybrid modeling of cross-flow filtration: Predicting the flux evolution and duration of ultrafiltration processes (opens in a new tab)

    Separation and Purification Technology 248, 117064, 2020 · In collaboration with BOKU · doi:10.1016/j.seppur.2020.117064

    Krippl M, Dürauer A, Duerkop M

  5. Hybrid Modeling for Simultaneous Prediction of Flux, Rejection Factor and Concentration in Two-Component Crossflow Ultrafiltration (opens in a new tab)

    Processes 8(12), 1625, 2020 · In collaboration with BOKU · doi:10.3390/pr8121625

    Krippl M, Dürauer A (and 2 more authors)

Further research

  1. A comprehensive antigen production and characterisation study for easy-to-implement, specific and quantitative SARS-CoV-2 serotests (opens in a new tab)

    EBioMedicine 67, 103348, 2021 · In collaboration with BOKU · doi:10.1016/j.ebiom.2021.103348

    Klausberger M, Grabherr R (and 5 more authors)

Preprints

  1. CFD-Informed Hybrid Modeling Unlocks Scalable, Tunable Amino Acid Production in Methanothermobacter marburgensis (opens in a new tab)

    bioRxiv, 2026.07.09.737395, 2026 · In collaboration with Arkeon · doi:10.64898/2026.07.09.737395

    Haslinger B, Rittmann SK-MR (and 11 more authors)

  2. Using hybrid modelling to predict growth of cyanobacterium Crocosphaera chwakensis CCY0110 and enhance Cyanoflan production across scales (opens in a new tab)

    SSRN, 7030018, 2026 · In collaboration with CyanoCare · doi:10.2139/ssrn.7030018

    Wucsits D, Mota R (and 8 more authors)

Novasign in other researchers’ papers

The authors thank Mark Duerkop, Benjamin Bayer and Roger Dalmau Díaz from Novasign GmbH for fruitful discussions about the iDoE concept.

Nold, Junghans et al., Boehringer IngelheimEngineering in Life Sciences, 2022 · 10.1002/elsc.202100123

Read the papers, then bring us a process

The papers say what the method did on the processes it was tested on. The next question is what it does on yours.

Book a demoSee the results overview